Thermo scientific revco blood bank refrigerators installation and operation 3 unpacking and inspection at delivery, examine the exterior for physical damage while the. This kit uses the highly processive thermo scientific phusion hot start ii highfidelity dna polymerase for exponential pcr amplification of dsdna plasmid to be mutated. Gallery covers a wide range of application areas, e. If necessary, use a temperature gradient to find the optimal annealing temperature for each templateprimer pair combination. By using the same innovative software for onboard and computer control, your instrument is always up to. Robust reactions maximal success with minimal optimization.
Thermo scientific phusion green highfidelity dna polymerase. Phusion highfidelity pcr master mix with gc buffer neb. Incorporating an exciting new technology, phusion dna polymerase brings together a novel pyrococcuslike enzyme with a processivityenhancing domain. Phusion highfidelity pcr master mix with hf buffer neb. All that is required is the addition of template, primers and water. Thermo scientific revco laboratory refrigerators and freezers unrivalled sample protection researchers worldwide protect more than two billion samples inside thermo scientific cold storage equipment. It combines the phusion dna polymerase and a reversibly bound, specific affibody ligand. Thermo scientific phusion highfidelity pcr master mix. Two optimized buffers are available for thermo scientific phusion highfidelity dna polymerases. Kit phusion highfidelity pcr kit product introduction ideal for high speed and highperformance pcr. All of our equipment is in excellent condition and will be fully tested to perform at factory specifications before being shipped. The product manual includes details for how to use the product, as well as details of its formulation and quality controls. Phusion highfidelity dna polymerase new england biolabs. Phusion highfidelity pcr master mix with gc buffer offers robust performance and can be used for all pcr applications.
Thermo scientific phusion buffers choose between two buffer packs to optimize highfidelity pcr or difficult or long template amplification using thermo scientific phusion highfidelity dna polymerases. Thermo scientific phusion highfidelity dna polymerase offers extreme performance for all major pcr applications. Thermo scientific phusion green hf buffer pack buffer packs for use in pcr with phusion highfidelity dna polymerases. Thermo scientific heratherm 11 1 safety notes basic operating precautions these operating instructions describe heratherm ovens. Thermo scientific phusion hot start ii dna polymerase 2 u. Trademarks thermo scientific is a brand owned by thermo fisher scientific inc. Easypure ii reservoir feed water purification system. Thermo scientific epijet bisulfite conversion kit is designed for simple and reliable bisulfite conversion of dna for methylation analysis. Pcr reaction setup for phusion thermo fisher scientific us.
Phusion hot start ii high fidelity dna polymerase is the most accurate hot start dna polymerase on the market. New england biolabs uk ltd phusion highfidelity pcr kit. Thermo scientific phusion sitedirected mutagenesis kit, point mutations, insertions and deletions can be introduced in any type of plasmid dna. The phusion highfidelity dna polymerase brings together a pyrococcuslike enzyme with a processivityenhancing domain. Fill in the volume of template dna you want to use, the number of pcr reactions you intend to run, and the individual reaction volume. Due to the unique nature of phusion dna polymerases, please pay special attention to the guidelines listed below. Thermo scientific revco blood bank refrigerators installation and operation 3 unpacking and inspection at delivery, examine the exterior for physical damage while the carriers representative is present. This calculator generates a pipetting table for setting up pcr reactions with thermo scientific phusion dna polymerases. The phusion dna polymerase generates long templates with an accuracy and. More details can be found in the technical manuals for phusion dna polymerases. Phusion highfidelity dna polymerase high performance for all. Browse by field and technique below, or use the search function above. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged.
Annealing rules for phusion dna polymerases are different from many common dna polymerases such as taq dna polymerases. Thermo scientific phusion green hf buffer pack phusion green. Phusion highfidelity pcr kit f553s 50 reactions in 50 l volume f553l 200 reactions in 50 l volume thermo scienti. Heratherm ovens have been manufactured to the latest state of the art and been tested. Thermo scientific phusion green hf buffer pack phusion. It uses a threestage deionization process combined with a 0. Shop online for a wide selection of fisher scientific isotemp programmable ovens offer exceptional performance, safe operation and precision control. Thermo scientific phusion highfidelity pcr kit 500u. Phusion dna polymerases thermo fisher scientific us. The thermo scientific phusion highfidelity dna polymerase offers high performance for all major pcr applications.
Installation and operation manual thermo scientific revco. The phusion highfidelity dna polymerase should be pipetted carefully and gently as the high glycerol content 50% in the storage buffer may otherwise lead to pipetting errors. Affibody inhibits the dna polymerase activity at room temperature and thus prevents the amplification of nonspecific products. Bioke has implemented multiple measures to keep our employees and business partners safe during the covid19 outbreak, and at the same time to maintain the continuity of supply of our products and support to our customers. The purchase price of this product includes a limited, nontransferable license under u. This product is manufactured by new england biolabs, inc. All trademarks mentioned in the operating instructions are the. Thermo scientific phusion highfidelity pcr kit life. Powerful software, cuttingedge technology and an array of accessories consistently delivers the high quality results you expect. Phusion dna polymerases offer robust performance with short protocol times, even in the presence of pcr inhibitors, and generate higher yields with lower enzyme amounts than other dna polymerase. Highperformancegravityconvection doubledoor chambervolume 2. Thermo scientific phusion green highfidelity dna polymerase 2 ul the most accurate thermostable dna polymerase available offering superior performance for cloning and other applications requiring high fidelity. Thermo scientific revco laboratory refrigerators and freezers.
Thermo scientific user manuals download manualslib. Phusion highfidelity dna polymerase is supplied with 5x phusion hf buffer, 5x phusion gc buffer, dmso and 50 mm mgcl2 solution. Thermo 2837 digital water bath the thermo 2837 digital water bath has a 12l capacity with a temperature range up to 99. Phusion highfidelity dna polymerase high performance for. Manufactured and qualitycontrolled at new england biolabs, thermo scientific phusion highfidelity. Thermo scientific gallery brings excellent analytical performance to colorimetric, enzymatic and electrochemical measurements in a compact and affordable design. Thermo scientific evolution 300 and 600 uvvis spectrophotometer user guide attachments thermo scientific evolution 300 and 600 uvvis spectrophotometer user guide 2 mb. Evolution 300600 uvvis spectrophotometer user guide.
Phusion dna polymerase was developed by finnzymes oy, now a part of thermo fisher scientific. Phusion dna polymerase 2 ul 5x phusion hf buffer 5x phusion gc buffer dmso 50 mm mgcl 2 solution store at 5 to 30c. Page 1 installation and operation manual thermo scientific thermolyne furnace benchtop industrial type fd1500m, type models. Both phusion hf buffer and phusion gc buffer provide 1. Phusion dna polymerase is supplied with standard 5x phusion hf buffer, as well as 5x phusion gc buffer, which can be used for complex or gcrich templates. Fd1530m, fd1530mcn, fd1530m33, fd1535m, fd1540m, fd1540mcn, fd1540m33, and fd1545m page 2 thermo fisher scientific. Since their introduction, thermo scientific phusion highfidelity dna polymerases have been referenced in thousands of publications for highperformance pcr and have become the choice for a multitude of demanding applications ranging from reconstruction, design and massivelyparallel, highthroughput sequencing of whole genomes.
Installation and operation manual thermo fisher scientific. Its unique structure, a novel pyrococcuslike enzyme fused with a processivityenhancing domain, increases. Phusion highfidelity pcr master mix with hf buffer is a 2x master mix consisting of phusion dna polymerase, deoxynucleotides and reaction buffer that has been optimized and includes mgcl 2. Hf buffer for phusion dna polymerases two optimized buffers are available for thermo scientific phusion highfidelity dna polymerases. It is a 2x master mix consisting of phusion dna polymerase, deoxynucleotides and reaction buffer that has been optimized and includes mgcl 2. Thermo cl2 manual mazda 323 c repair manual productspecifications thermo scientific cl2 1999 jeep wrangler sahara manual thermo iec centrifuges and centra cl3r and pdf manual thermo scientific cl2 manuals suzuki z250 quad sport manual thermo scientific manuals biosurplus. Datasheet for phusion highfidelity dna polymerase m0530. Manufactured and qualitycontrolled at new england biolabs, thermo scientific phusion highfidelity dna polymerase offers both high fidelity and robust performance. Due to the nature of the phusion highfidelity dna polymerase, the optimal reaction conditions may differ from pcr protocols for standard dna polymerases.
Phusion dna polymerase in phusion hf buffer is determined to be 4. Thermo scientific easypure ii rf 21 section 2 introduction the thermo scientific easypure ii rf is a batchfed water purification system designed to provide type i reagentgrade water with extremely low organic carbon content. Product information thermo scientific phusion site. Thermo 2837 digital water bath marshall scientific.
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